Catalogue
Swine anti Mouse IgG (heavy and light chains)
Catalog number: SwAM/IgG(H+L)| Clone | Polyclonal |
| Product Type |
Secondary Antibodies Unpurified polyclonal antibody |
| Units | 1 ml |
| Host | Swine |
| Species Reactivity |
Mouse |
| Application |
Immunoelectrophoresis Precipitation assays |
Background
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in mouse serum, as tested by immunoelectrophoresis.
Potential uses: In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in mouse serum; as bridging reagent in a peroxidase antiperoxidase technique using M/PAP; to prepare an immunoadsorbent for the production of Ig-free mouse serum, plasma or non-Ig preparations.
Source
Purified normal IgG isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Product
Neat liquid antiserum containing 0.05% sodium azide as a preservative.
Formulation: Neat liquid antiserum containing 0.05% sodium azide as a preservative.
Specificity
Precipitating polyclonal swine antiserum to mouse IgG, heavy and light chains.
Species Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Applications
Precipitation assays. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Storage
4°C short term, - 20°C long term.
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Safety Datasheet(s) for this product:
| NM_Sodium Azide |