Catalogue
Sheep anti Rat SSeCKS - myristylated (PKC) substrate
Catalog number: S125P| Isotype | IgG |
| Product Type |
Polyclonal Antibody |
| Units | 250 µg |
| Host | Sheep |
| Species Reactivity |
Rat |
| Application |
Immunohistochemistry Immunoprecipitation Western Blotting |
Background
Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation and proliferation, yet little is known about downstream substrates or their roles in normal or diseased states. SSeCKS, a novel protein kinase C substrate originally isolated as a src-suppressed negative mitogenic regulator in fibroblasts, controls actinbased cytoskeletal architecture and scaffolds key signaling kinases such as protein kinase C and protein kinase A. Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation. A role for SSeCKS, a PKA/PKC scaffolding protein, has been implicated during the process of spermiogenesis and in the actin-based stellate morphology of mesangial cells
Synonyms: SSeCKS; A-kinase anchor protein 12; AKAP12; src-suppressed C kinase substrate
Source
Sheep were immunized with a recombinant protein corresponding to amino acids 620-1596 of the SSeCKS protein.
Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form: Unconjugated
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Antibody can be used for Western blot (2-5 µg/ml), immunohistochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Rat aortic smooth muscle
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. Lin, X., et al. 'A novel src- and ras-suppressed protein kinase C substrate with cytoskeletal architecture.' J. Biol. Chem. 1996, 271, 28430-28438
2. Siegel, S.M., et al. 'SSeCKS immunolabeling in rat primary sensory neurons.' Brain Res. 2002, 926, 126-136
3. Nelson, P.J., et al. 'Involvement of the protein kinase C substrate, SSeCKS, in the actin-based stellate morphology of mesangial cells.' J. Cell. Sci. 1999, 112, 361-370
Protein Reference(s)
Database Name: UniProt
Accession Number: Q62766 (Rat)
Species Accession: Rat
Safety Datasheet(s) for this product:
| NM_Sodium Azide |
|
Western blotting total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyze. The protein was transferred to a PVDF membrane, blocked 1XTBS, 0.1% tween 20/ 5%NFDM, probed with 5 µg/ml of pre-immune serum 5 ug of S125P antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-sheep IgG coupled to HRP 1:10,000, and detected with luminol. |
Western blotting total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyze. The protein was transferred to a PVDF membrane, blocked 1XTBS, 0.1% tween 20/ 5%NFDM, probed with 5 µg/ml of pre-immune serum 5 ug of S125P antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-sheep IgG coupled to HRP 1:10,000, and detected with luminol.