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Sheep anti Rat ADAR2 (Adenosine Deaminase Acting on RNA 2)

Catalog number: U115P
Isotype IgG
Product Type Polyclonal Antibody
Units 50 µg
Host Sheep
Species Reactivity Rat
Application Western Blotting

Background
RED 1 also called ADAR2 is involved in the editing of the messenger RNAs for glutamate receptor (GluR) subunits by site-selective adenosine deamination. It edits both the GluR-B Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently.

Synonyms: RED-1, RED1; ADAR2, DRADA2 dsRNA adenosine deaminase, RNA-editing deaminase 1, RNA-editing enzyme 1

Source
Sheep were immunized with a GST fusion protein containing amino acid sequence 6-66 of rat RED1

Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product Form: Unconjugated

Formulation: .

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Suggested dilution for WB: 1:1000. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Rat brain

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

Protein Reference(s)

Database Name: UniProt

Accession Number: P51400 (Rat)

Species Accession: Rat

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: U115P Categories : Primary antibodies, Primary antibodies, Polyclonal Antibody
Figure 1. Immunofluorescence staining of mouse anterior horns of spinal cord at 6 months, 15 months and 26 months of age. Antibody used at 1:100 dilution and visualized using donkey anti-sheep conjugated to Alexa 555 dye (red color). The blue color is a fluorescent nuclear probe, TOPRO3. (Image modified from original in citation 1). Figure 1. Immunofluorescence staining of mouse anterior horns of spinal cord at 6 months, 15 months and 26 months of age. Antibody used at 1:100 dilution and visualized using donkey anti-sheep conjugated to Alexa 555 dye (red color). The blue color is a fluorescent nuclear probe, TOPRO3. (Image modified from original in citation 1).
Figure 1. Immunofluorescence staining of mouse anterior horns of spinal cord at 6 months, 15 months and 26 months of age. Antibody used at 1:100 dilution and visualized using donkey anti-sheep conjugated to Alexa 555 dye (red color). The blue color is a fluorescent nuclear probe, TOPRO3. (Image modified from original in citation 1).

Figure 1. Immunofluorescence staining of mouse anterior horns of spinal cord at 6 months, 15 months and 26 months of age. Antibody used at 1:100 dilution and visualized using donkey anti-sheep conjugated to Alexa 555 dye (red color). The blue color is a fluorescent nuclear probe, TOPRO3. (Image modified from original in citation 1).