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Sheep anti Human Vav-2 DPH

Catalog number: S120P
Clone Polyclonal
Isotype IgG
Product Type Polyclonal Antibody
Units 250 µg
Host Sheep
Species Reactivity Human
Application Immunoprecipitation
Western Blotting

Background
The immunogen used for the anti-human Vav-2 DPH region was a his tagged fusion protein Vav-2 corresponding to amino acid sequence 287-578 and having a molecular weight of 100kD. Vav-2 has a molecular weight of 100 kD and shares over a 50% homology at the amino acid and nucleic acid level with Vav. Vav-2 is a ubiquitously expressed structural homolog of the Vav protooncogene that is expressed preferentially in hematopoetiic cells. Both proteins are comprised of a Dbl- homology ((DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type vav-2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a Vav-2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokenesis frequently are uncoupled.

Synonyms: VAV-2; Guanine nucleotide exchange factor VAV-2, Dbl homology (DH) domain

Source
Sheep were immunized with a his tagged fusion protein Vav-2 corresponding to amino acid sequence 287-578 and having a molecular weight of 100kD

Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product Form: Unconjugated

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Western Blot 1:2500 -1:5000. Immunoprecipitation -Empirical Dilutions should be titrated for each application to obtain the optimal antibody titer.

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

Protein Reference(s)

Database Name: UniProt

Accession Number: P52735 (Human)

Species Accession: Human

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: S120P Categories : Primary antibodies, Primary antibodies, Polyclonal Antibody
Legend: HEK293 cells were transfected with either emply expression vector (pSR␣ ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 ul/300ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 ul/300ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 ul/300ug lysate). DPH or SHD anti Vav2 antisera (2.5ul/300ug lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000. Legend: HEK293 cells were transfected with either emply expression vector (pSR␣ ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 ul/300ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 ul/300ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 ul/300ug lysate). DPH or SHD anti Vav2 antisera (2.5ul/300ug lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000.
Legend: HEK293 cells were transfected with either emply expression vector (pSR␣ ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 ul/300ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 ul/300ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 ul/300ug lysate). DPH or SHD anti Vav2 antisera (2.5ul/300ug lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000.

Legend: HEK293 cells were transfected with either emply expression vector (pSR␣ ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 ul/300ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 ul/300ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 ul/300ug lysate). DPH or SHD anti Vav2 antisera (2.5ul/300ug lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000.