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Sheep anti Human TAK1

Catalog number: X1088P
Isotype IgG
Product Type Polyclonal Antibody
Units 250 µg
Host Sheep
Species Reactivity Human
Application Immunoprecipitation
Western Blotting

Background
C-TAK1 (Cdc25C associated protein kinase) is a ubiquitously expressed protein kinase found in human tissues and cell lines and can phosphorylate Cdc25C on serine 216 in vitro. C-TAK1 is distinct from the DNA damage checkpoint kinase Chk1 and may regulate interactions between Cdc25C and 14-3-3 in vivo by phosphorylating Cdc25C at serine 216.

Synonyms: Mark3, Sheep anti-Cdc25c associated protein kinase-1, human

Source

Immunogen: Recombinant, full-length His-tagged human C-TAK1 produced in E. Coli.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody detects c-tak1 by Western blot analysis at 1 to 5 µg/ml. Detects c-tak1 in RKO and HCT116 cell lysates. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Recombinant His-tagged C-TAK1 protein

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Peng, C.Y., et al. C-TAK1 protein kinase phosphorylates human Cdc25C on serine 216 and promotes 14-3-3 protein binding. Cell Growth Differ. 1998, 9, 197-208 2. Ogg, S., et al. Purification of a serine kinase that associates with and phosphorylates human Cdc25C on serine 216. J. Biol. Chem. 1994, 269, 30461-30469 3. Busby, E.C., et al. The radiosensitizing agent 7-hydroxystaurosporine (UCN-01) inhibits the DNA damage checkpoint kinase hChk1. Cancer Res. 2000, 60, 2108-2112

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: X1088P Categories : Primary antibodies, Primary antibodies, Polyclonal Antibody
"Western blot analysis using c-tak1 antibody (Cat. No. X1088P) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3)." "Western blot analysis using c-tak1 antibody (Cat. No. X1088P) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3)."
"Western blot analysis using c-tak1 antibody (Cat. No. X1088P) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3)."

"Western blot analysis using c-tak1 antibody (Cat. No. X1088P) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3)."