Sheep anti Human CalreticulinCatalog number: Z100P
Calreticulin, a Ca2+ chaperone, is an abundant highly conserved calcium binding protein found in the lumen of the endoplasmic reticulum of eukaryotic cells. This lumen protein can be used to measure the intactness of membrane preparations. It is used as a marker for subcellular fractionation of cells.
Sheep were immunized with a recombinant protein derived from the carboxy terminus of the calreticulin protein fused to KLH.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Antibody can be used for Western blotting (1:1000-1:5000 dilution) and immunofluorescence (1:50 dilution). Optimal concentration should be evaluated by serial dilutions. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.
Functional Analysis: Western Blotting
Positive Control: MDCK cells; MCF-7 cells.
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Kageyama, S., et al. 'Urinary calreticulin in the diagnosis of bladder urothelial carcinoma.' Int. J. Urol. 2009, 16, 481-486 2. Silerova, M., et al. 'Characterization, molecular cloning and localization of calreticulin in Eisenia fetida earthworms.' Gene 2007, 397, 169-177 3. Souto-Padron, T., et al. 'Immunocytochemical localisation of calreticulin in Trypanosoma cruzi.' Histochem. Cell Biol. 2004, 122, 563-569
Database Name: UniProt
Accession Number: Q6IAT4 (Human)P14211 (Mouse)P18418 (Rat)P28490
Species Accession: Human, Rat, Mouse
Safety Datasheet(s) for this product:
|Western blot using antibody at 1:5000 dilution on indicated samples. Immunofluorescence of MCF-7 cells stained with antibody at 1:50 dilution.|
Western blot using antibody at 1:5000 dilution on indicated samples. Immunofluorescence of MCF-7 cells stained with antibody at 1:50 dilution.