Catalogue

Background
Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.

Synonyms: Perlecan, HSPG

Source
A7L6 is a Rat monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a Fisher Rat immunized with high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.

Product
The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.

Formulation: The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.

Specificity
A7L6 recognizes domain IV of the core protein of the large heparan sulphate proteoglycan or perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment.

Applications
A7L6 is useful for immunoprecipitation, immunoblotting, immunocytochemistry and immuno-histochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Shipping Conditions: Ship at ambient temperature.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Couchman, J.R. (1987). Heterogeneous distribution of a basement membrane heparin sulfate proteoglycan in Rat tissues. The Journal of Cell Biology 105, 1901-16. 2. Couchman, J. R., and Ljubimov, A. V. (1989). Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies. Matrix 9, 311-21. 3. Horiguchi, Y., Couchman, J. R., Ljubimov, A. V., Yamasaki, H., and Fine, J. D. (1989). Distribution, ultrastructural localization, and ontogeny of the core protein of a heparan sulfate proteoglycan in Human skin and other basement membranes. 4. J Histochem Cytochem 37, 961-70. Ljubimov, A. V., Bartek, J., Couchman, J. R., Kapuller, L. L., Veselov, V. V., Kovarik, J., Perevoshchikov, A. G., and Krutovskikh, V. A. (1992). Distribution of individual components of basement membrane in Human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer 50, 562-66. 5. Couchman, J. R., Ljubimov, A. V., Sthanam, M., Horchar, T., and Hassell, J. R. (1995). Antibody mapping and tissue localization of globular and cysteine-rich regions of perlecan domain III. J Histochem Cytochem 43, 955-63. 6. Tapanadechopone, P., Hassell, J. R., Rigatti, B., and Couchman, J. R. (1999). Localization of glycosaminoglycan substitution sites on domain V of Mouse perlecan. Biochem Biophys Res Commun 265, 680-90. 7. Tingbø, M. G., Kolset, S. O., Ofstad, R., Enersen, G., Hannesson, K. O. (2006). IdentifiCation and distribution of heparan sulfate proteoglycans in the white muscle of Atlantic cod (Gadus morhua) and spotted wolffish (Anarhichas minor). Comparative Biochemistry and Physiology Part B 143,441-52. 8. Hayes AJ, Shu CC, Lord MS, Little CB, Whitelock JM, Melrose J. Pericellular colocalisation and interactive properties of type VI collagen and perlecan in the intervertebral disc. Eur Cell Mater. 2016 Jul 5;32:40-57. 9. Furuta GT, Dzus AL, Taylor CT, Colgan SP. Parallel induction of epithelial surface-associated chemokine and proteoglycan by cellular hypoxia: implications for neutrophil activation. J Leukoc Biol. 2000 Aug;68(2):251-9.

Safety Datasheet(s) for this product: