Catalogue

Background
In immunoelectrophoresis against mouse milk precipitation of not less than 7 different proteins may be obtained. However the number of visible precipitin lines varies among individual specimens of mouse milk. The total concentration of protein, their proportion of the different protein components change markedly during the course of lactation. There are also strain differences in the protein composition, but differences among individual animals tend to be greater. A protein detected in a mouse secretion by this antiserum is of secretory origin. However an analogous or homologous protein may be present also n other kind of secretions (e.g. saliva). Not all precipitable proteins have been identified. Known proteins include free secretory component and lactoferrin. In immunoelectrophoresis to identify the milk protein pattern, to compare individual samples and to make comparisons with serum protein patterns or those of other secretions; to identify individual components, their possible origin and significant changes in concentration. To test the purity of an isolated milk protein..

Source
Pooled mouse milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Product
Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.

Formulation: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.

Specificity
Precipitating polyclonal Rabbit antiSerum to Mouse milk-specific proteins

Species Reactivity: Inter-species cross reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiSerum has not been adsorbed for such cross reactivity. Consequently it is not speciesspecific.

Applications
Precipitation assays. In immunoelectrophoresis use 2 μl milk or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl milk samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual protein components in individual milk samples. Immunohistochemical application: For immunostaining, paraffin-embedded sections are dehydrated and antigenic epitopes are exposed by treatment with 10mM citrate buffer for 5min at 121°C using an autoclave (see reference 1).

Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Mizuki Yamamoto, Chiho Abe, Sakura Wakinaga, Kota Sakane, Yo Yumiketa, Yuu Taguchi, Takayuki Matsumura, Kosuke Ishikawa, Jiro Fujimoto, Kentaro Semba, Maki Miyauchi, Taishin Akiyama & Jun-ichiro Inoue TRAF6 maintains mammary stem cells and promotes pregnancy-induced mammary epithelial cell expansion Communications Biology volume 2, Article number: 292 (2019) DOI - https://doi.org/10.1038/s42003-019-0547-7