Catalogue
Rabbit anti Human SPT16 NT
Catalog number: X1583P| Isotype | IgG |
| Product Type |
Polyclonal Antibody |
| Units | 100 µg |
| Host | Rabbit |
| Species Reactivity |
Human Mouse |
| Application |
Western Blotting |
Background
SPT16 and SSRP1 are found in all eurkaryotic cells and exists as part of an essential heterodimer complex. Together, SPT16 and SSRP1 are a highly abundant nuclear complex that in mammals has been called FACT, or Facilitates Chromatin Transcription. FACT enhances the processivity of RNA polymerase II through nucleosomes. Evidence also points to a role for FACT in replication, possibly by enhancing DNA polymerase through nucleosomes as well.
Source
Immunogen: Recombinant protein consisting of amino acids 1-329 of the human SPT16 protein conjugated to KLH.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Recombinant proteins conjugated to OVA, GST, His tags and other.
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. Keller, D.M., et al. 'A DNA damage-induced p53 serine 392 kinase complex contains CK2, hSPT16 and SSRP1.' Mol. Cell Biol. 2001, 7, 283-292
Protein Reference(s)
Database Name: SwissProt
Accession Number: Q08945
Species Accession: Human
Safety Datasheet(s) for this product:
| NM_Sodium Azide |