Catalogue
Rabbit anti Human Sphingosine Kinase 2, Long Form
Catalog number: X1775P| Product Type |
Polyclonal Antibody |
| Units | 100 µg |
| Host | Rabbit |
| Species Reactivity |
Human |
| Application |
ELISA Western Blotting |
Background
The long form of sphingosine kinase 2 (Sphk2-L) is a species-specific isoform of the Sphk2 enzyme expressed in human but not in mouse. It is an N-terminal extended form of Sphk2, extended by 36 amino acids. It functions as expected, catalyzing the formation of sphingosine 1-phosphate from sphingosine, however it appears to have a decreased ability to inhibit DNA synthesis as compared to Sphk2-S (short form of Sphk2). Under normal cellular conditions, Sphk2-L does not appear to inhibit DNA synthesis, but under serum deprivation, SphK2-L translocates to the nucleus and accumlates. This accumlation may be involved in the cessation of cell proliferation or apoptosis depending on the cell type.
Synonyms: Sphk2-L, N-terminal-extended Sphingosine Kinase 2
Source
Immunogen: Synthetic peptide derived from the N-terminal extension of the Sphk2-L protein.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Antibody can be used for Western blotting (1-5 µg/ml) and ELISA. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting, ELISA
Positive Control: HEK293 cells
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. Okada, T., et al. 'Involvement of N-terminal-extended form of sphingosine kinase 2 in serum-dependent regulation of cell proliferation and apoptosis.' J. Biol. Chem. 2005, 280, 36318-36325
Protein Reference(s)
Database Name: UniProt
Accession Number: AF245447
Species Accession: Human
Safety Datasheet(s) for this product:
| NM_Sodium Azide |