Rabbit anti Human Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT
Catalog number: X1093P| Isotype | IgG |
| Product Type |
Polyclonal Antibody |
| Units | 100 µg |
| Host | Rabbit |
| Species Reactivity |
Human Mouse Rat |
| Application |
Western Blotting |
Background
EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocyte- containing and other tissues.
Synonyms: Endothelial cell differentiation gene 1 C-terminal
Source
Immunogen: Synthetic peptide derived from the C-terminal of the EDG-1 receptor
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Detects EDG1 receptors at concentration of 5-10 µg/ml by Western blot using a human EDG1 receptor transfected cell line. Detects an approximately 44 kDa band in RH7777 cells transfected with full length human EDG1. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: RH7777 cells transfected with full length EDG-1 protein.
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. Edg-1, the G protein-coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation. Liu, Y., et al., J. Clin. Invest., 2000, 106, 951-961.
2. Expression and characterization of edg-1 receptors in rat cardiomyocytes calcium deregulation in response to sphingosine-1-phosphate. Nakajima, N., et al., Eur. J. Biochem., 2000, 267, 5679-5686.
3. Diversity of cellular receptors and functions for the lysophospholipid growth factors lysophosphatidic acid and sphingosine 1-phosphate. Goetzl, E.J. and An, S.,FASEB J., 12(15), 1589-1598 (1998).
Protein Reference(s)
Database Name: SwissProt
Accession Number: P21453
Species Accession: Human
Safety Datasheet(s) for this product:
| NM_Sodium Azide |
|
Western blot analysis of lysates of RH7777 cells transfected with full lenth human EDG-1 protein using anti-EDG-1 CT antibody at 10 µg/ml (1), antibody preincubated with specific blocking peptide (2) and antibody preincubated with non-specific control peptide (3) using Pierce Femto Signal substrate. |
Western blot analysis of lysates of RH7777 cells transfected with full lenth human EDG-1 protein using anti-EDG-1 CT antibody at 10 µg/ml (1), antibody preincubated with specific blocking peptide (2) and antibody preincubated with non-specific control peptide (3) using Pierce Femto Signal substrate.