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Rabbit anti Human Sphingomyelin Phosphodiesterase 3

Catalog number: X2360P
Product Type Polyclonal Antibody
Units 100 µg
Host Rabbit
Species Reactivity Human
Application Western Blotting

Background
Sphingomyelin phosphodiesterase 3 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these two cellular events independently. It also hydrolyzes sphingosylphosphocholine. Sphingomyelin phosphodiesterase 3 regulates the cell cycle by acting as a growth suppressor in confluent cells. It likely acts as a regulator of postnatal development and participates in bone and dentin mineralization. It is activated by unsaturated fatty acids and phosphatidylserine. it is predominantly expressed in the brain.

Synonyms: nSMase2; Neutral sphingomyelinase 2; EC 3.1.4.12

Source

Immunogen: Synthetic peptide derived from human sphingomyelin phosphodiesterase 3

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1-5 µg/ml starting dilution). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Predominantly expressed in the brain.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Hofmann, K., et al. Cloning and characterization of the mammalian brain-specific, Mg2+-dependent neutral sphingomyelinase.; Proc. Natl. Acad. Sci. U.S.A. 97:5895-5900(2000). 2. Miura, Y., et al. Hydrolysis of sphingosylphosphocholine by neutral sphingomyelinases.; FEBS Lett. 557:288-292(2004). 3. Marchesini, N., et al. Role for mammalian neutral sphingomyelinase 2 in confluence-induced growth arrest of MCF7 cells.; J. Biol. Chem. 279:25101-25111(2004).

Protein Reference(s)

Database Name: UniProt

Accession Number: Q53YV1 (Human)

Species Accession: Human

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: X2360P Categories : Primary antibodies, Primary antibodies, Polyclonal Antibody
" Western blot using nSMase2 antibody (Cat. No. X2360P) on human brain lysate (Cat. No. X1633C). Lysate used at 14 µg/lane. Antibody used at 10 µg/ml. Secondary antibody, mouse anti-rabbit HRP (Cat. No. X1207M), used at 1:50k dilution. " " Western blot using nSMase2 antibody (Cat. No. X2360P) on human brain lysate (Cat. No. X1633C). Lysate used at 14 µg/lane. Antibody used at 10 µg/ml. Secondary antibody, mouse anti-rabbit HRP (Cat. No. X1207M), used at 1:50k dilution. "
" Western blot using nSMase2 antibody (Cat. No. X2360P) on human brain lysate (Cat. No. X1633C). Lysate used at 14 µg/lane. Antibody used at 10 µg/ml. Secondary antibody, mouse anti-rabbit HRP (Cat. No. X1207M), used at 1:50k dilution. "

" Western blot using nSMase2 antibody (Cat. No. X2360P) on human brain lysate (Cat. No. X1633C). Lysate used at 14 µg/lane. Antibody used at 10 µg/ml. Secondary antibody, mouse anti-rabbit HRP (Cat. No. X1207M), used at 1:50k dilution. "