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Rabbit anti Human Sodium/hydrogen exchanger 1

Catalog number: X2338P
Product Type Polyclonal Antibody
Units 100 µg
Host Rabbit
Species Reactivity Human
Application Western Blotting

Background
NHE-1 is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. NHE-1 plays an important role in signal transduction. Located in the kidney and intestine.

Synonyms: Sodium/hydrogen exchanger 1; NHE-1; SLC9A1; Solute carrier family 9 member 1

Source

Immunogen: Synthetic peptide derived from the human NHE-1 protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Protein expressed in kidney and intestine.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Pang, T., et al. 'Expression of calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger.; J. Biol. Chem. 277:43771-43777(2002). 2. Slepkov, E.R., et al. 'Proline residues in transmembrane segment IV are critical for activity, expression and targeting of the Na+/H+ exchanger isoform 1.; Biochem. J. 379:31-38(2004). 3. Slepkov, E.R., et al. 'Structural and functional characterization of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger.; J. Biol. Chem. 280:17863-17872(2005).

Protein Reference(s)

Database Name: UniProt

Accession Number: P19634

Species Accession: Human

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: X2338P Categories : Primary antibodies, Primary antibodies, Polyclonal Antibody
Western blot using NHE1 antibody (Cat. No. X2338P) on HT-29 cell lysate (Cat. No. X1633C). Lysate loaded at 30 µg/lane. Antibody used at 10 µg/ml. Secondary antbody, mouse anti-rabbit HRP (Cat. No. X1207M), used at 1:150k dilution. Western blot using NHE1 antibody (Cat. No. X2338P) on HT-29 cell lysate (Cat. No. X1633C). Lysate loaded at 30 µg/lane. Antibody used at 10 µg/ml. Secondary antbody, mouse anti-rabbit HRP (Cat. No. X1207M), used at 1:150k dilution.
Western blot using NHE1 antibody (Cat. No. X2338P) on HT-29 cell lysate (Cat. No. X1633C). Lysate loaded at 30 µg/lane. Antibody used at 10 µg/ml. Secondary antbody, mouse anti-rabbit HRP (Cat. No. X1207M), used at 1:150k dilution.

Western blot using NHE1 antibody (Cat. No. X2338P) on HT-29 cell lysate (Cat. No. X1633C). Lysate loaded at 30 µg/lane. Antibody used at 10 µg/ml. Secondary antbody, mouse anti-rabbit HRP (Cat. No. X1207M), used at 1:150k dilution.