Catalogue

Background
Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins. Interacts with EPHA2, VAV1, LCP2 and PDGFRB. The C-terminal domain is essential for the homodimerization and the interaction with CBL. While the interaction with CBL is apparently mediated via the hydrophobic region of this domain, the highly charged region is apparently required for the homodimerization. Expressed in lung and fetal brain. Weakly expressed in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas.

Source

Immunogen: Synthetic peptide derived from the human SLAP1 protein.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Dragone, L.L., et al. 'Src-like adaptor protein (SLAP) regulates B cell receptor levels in a c-Cbl-dependent manner.' Proc. Natl. Acad. Sci. USA 2006, 103(48):18202-18207 2. Dragone, L.L., et al. 'SRC-like adaptor protein regulates B cell development and function.' J. Immunol. 2006, 176(1):335-345 3. Myers, M.D., et al. 'Src-like adaptor protein down-regulates T cell receptor (TCR)-CD3 expression by targets TCRzeta for degradation.' J. Cell. Biol. 2005, 170(2):285-294

Protein Reference(s)

Database Name: UniProt

Accession Number: Q13239

Species Accession: Human

Safety Datasheet(s) for this product: