Rabbit anti Human Ribonucleoside-diphosphate reductase, large subunit (RRM1)
Catalog number: X2735P| Isotype | Ig |
| Product Type |
Antigen Immunoaffinity Purified Polyclonal |
| Units | 100 µg |
| Host | Rabbit |
| Species Reactivity |
Human |
| Application |
ELISA Immunohistochemistry Western Blotting |
Background
This protein is one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in the S phase of dividing cells. The gene for RRM1 is, along with several others, located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region are associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. RRM1 plays an important role in malignancies and disease that involve this region.
Synonyms: Ribonucleoside-diphosphate reductase subunit M1, EC=1.17.4.1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleotide reductase large subunit, RRM1, RR1
Source
Immunogen: Synthetic peptide derived from the human RRM1 protein
Product
Product Form: Affinity Purified
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Antigen Immunoaffinity Purification
Concentration: See vial for concentration
Applications
This antibody can be used for immunohistochemistry on formalin/paraffin sections (10-15 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Immunohistochemistry
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Protein Reference(s)
Database Name: SwissProt
Accession Number: P23921
Species Accession: Human
Safety Datasheet(s) for this product:
| NM_Sodium Azide |
|
Immunohistochemical staining of human liver tissue diagnosed with hepatocellular carcinoma using RRM1 antibody (Cat. No. X2735P) at 15 µg/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain. |
Immunohistochemical staining of human liver tissue diagnosed with hepatocellular carcinoma using RRM1 antibody (Cat. No. X2735P) at 15 µg/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain.