Rabbit anti Human PH domain leucine-rich repeat-containing protein phosphatase 2
Catalog number: X2737P| Isotype | Ig |
| Product Type |
Antigen Immunoaffinity Purified Polyclonal |
| Units | 100 µg |
| Host | Rabbit |
| Species Reactivity |
Human |
| Application |
ELISA Immunohistochemistry Western Blotting |
Background
PHLPP2 is a protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.
Synonyms: PHLPP2, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like; PHLPP-like
Source
Immunogen: Synthetic peptide derived from the human PHLPP2 protein
Product
Product Form: Pure Affinity Purified
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Antigen Immunoaffinity Purification
Concentration: See vial for concentration
Applications
This antibody can be used for immunohistochemistry on formalin/paraffin sections (10-15 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Immunohistochemistry
Positive Control: Normal human kidney
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Protein Reference(s)
Database Name: SwissProt
Accession Number: Q6ZVD8
Species Accession: Human
Safety Datasheet(s) for this product:
| NM_Sodium Azide |
|
Immunohistochemical staining of normal human kidney tissue using PHLPP2 antibody (Cat. No. X2737P) at 15 µg/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain. |
Immunohistochemical staining of normal human kidney tissue using PHLPP2 antibody (Cat. No. X2737P) at 15 µg/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain.