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Rabbit anti Human Nucleolar Protein 4

Catalog number: X2417P
Isotype IgG
Product Type Polyclonal Antibody
Units 100 µg
Host Rabbit
Species Reactivity Human
Application ELISA
Immunohistochemistry
Western Blotting

Background
Novel methylation target specific for cervical cancer. Could be a marker in the early detection, risk prediction and etiologic research of cervial cancer. While the biological role of NOL4 in cervical cancer has yet to be determined, it is possible that it could be a potential tumor suppressor gene. It also appears to have important roles in cell cycle and apoptosis as well as functioning in ribosomal transcription and subunit assembly.

Synonyms: NOL4

Source

Immunogen: Synthetic peptide derived from the human NOL4 protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1-5 µg/ml) and immunohistochemistry on paraffin embedded tissues (10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Ueki, N., et al. 'NOLP: identification of a novel human nucleolar protein and determination of sequence requirements for its nucleolar localization.' Biochem Biophys Res Commun 1998;252:97-102. 2. Wang, S.S., et al. 'Identification of novel methylation markers in cervical cancer using restriction landmark genomic scanning.' Cancer Res 2008;68:2489-2497. 3. Visintin R, et al. 'The nucleolus: the magician's hat for cell cycle tricks.' Curr Opin Cell Biol 2000;12:372–7. 4. Olson MO, et al. 'The nucleolus: an old factory with unexpected capabilities.' Trends Cell Biol 2000;10:189–96.

Protein Reference(s)

Database Name: UniProt

Accession Number: O94818-Human

Species Accession: Human

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: X2417P Categories : Primary antibodies, Primary antibodies, Polyclonal Antibody
Staining on paraffin embedded normal human kidney sections using NOL4 antibody (Cat. No. X2417P) at 10 µg/ml. Antigen retrieval used: 10 mM Na Citrate pH 6.0, 10 minutes pressure cooker method, developed with anti rabbit HRP and DAP substrate. Counterstained with methyl blue. Staining on paraffin embedded normal human kidney sections using NOL4 antibody (Cat. No. X2417P) at 10 µg/ml. Antigen retrieval used: 10 mM Na Citrate pH 6.0, 10 minutes pressure cooker method, developed with anti rabbit HRP and DAP substrate. Counterstained with methyl blue.
Staining on paraffin embedded normal human kidney sections using NOL4 antibody (Cat. No. X2417P) at 10 µg/ml. Antigen retrieval used: 10 mM Na Citrate pH 6.0, 10 minutes pressure cooker method, developed with anti rabbit HRP and DAP substrate. Counterstained with methyl blue.

Staining on paraffin embedded normal human kidney sections using NOL4 antibody (Cat. No. X2417P) at 10 µg/ml. Antigen retrieval used: 10 mM Na Citrate pH 6.0, 10 minutes pressure cooker method, developed with anti rabbit HRP and DAP substrate. Counterstained with methyl blue.