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Rabbit anti Human Lass4

Catalog number: X2300P
Product Type Polyclonal Antibody
Units 100 µg
Host Rabbit
Species Reactivity Human
Application Western Blotting

Background
It is thought that Lass4 may be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donnors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner.

Source

Immunogen: Synthetic peptide derived from human Lass4 protein.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1 mg/ml starting dilution). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Lung and embryo

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Min, J., et al. '(Dihydro)ceramide synthase 1 regulated sensitivity to cisplatin is associated with the activation of p38 mitogen-activated protein kinase and is abrogated by sphingosine kinase 1.' Mol. Cancer Res. 2007, 5(8):801-812 2. Ruckhaberle, E., et al. 'Microarray analysis of altered sphingolipid metabolism reveals prognostic significance of sphingosine kinase 1 in breast cancer.' Breast Cancer Res Treat. 2007 Dec 4 [Epub ahead of print]

Protein Reference(s)

Database Name: UniProt

Accession Number: Q9HA82

Species Accession: Human

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: X2300P Categories : Primary antibodies, Primary antibodies, Polyclonal Antibody
" Western blot analysis using LAG1 longevity assurance homolog 4 (Cat.# X2298P) at 0.75ug/ml on human lung lysate14 ug/lane. Lane A] conjugate alone, Lane B] antibody plus 27 ug blocking peptide (Cat. # X2299B ), Lane C] antibody alone. Visualized using Pierce West Femto substrate system. Anti Rabbit secondary used at 1:3.5K dilution (Cat. # X1207M). Exposure for 5 minutes" " Western blot analysis using LAG1 longevity assurance homolog 4 (Cat.# X2298P) at 0.75ug/ml on human lung lysate14 ug/lane. Lane A] conjugate alone, Lane B] antibody plus 27 ug blocking peptide (Cat. # X2299B ), Lane C] antibody alone. Visualized using Pierce West Femto substrate system. Anti Rabbit secondary used at 1:3.5K dilution (Cat. # X1207M). Exposure for 5 minutes"
" Western blot analysis using LAG1 longevity assurance homolog 4 (Cat.# X2298P) at 0.75ug/ml on human lung lysate14 ug/lane. Lane A] conjugate alone, Lane B] antibody plus 27 ug blocking peptide (Cat. # X2299B ), Lane C] antibody alone. Visualized using Pierce West Femto substrate system. Anti Rabbit secondary used at 1:3.5K dilution (Cat. # X1207M). Exposure for 5 minutes"

" Western blot analysis using LAG1 longevity assurance homolog 4 (Cat.# X2298P) at 0.75ug/ml on human lung lysate14 ug/lane. Lane A] conjugate alone, Lane B] antibody plus 27 ug blocking peptide (Cat. # X2299B ), Lane C] antibody alone. Visualized using Pierce West Femto substrate system. Anti Rabbit secondary used at 1:3.5K dilution (Cat. # X1207M). Exposure for 5 minutes"