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Rabbit anti Human Lass3

Catalog number: X2304P
Product Type Antigen Immunoaffinity Purified Polyclonal
Units 100 µg
Host Rabbit
Species Reactivity Human
Application Western Blotting

Background
LASS3 (Longevity Assurance homolog 3) is a member of a highly conserved family of proteins from yeast to humans which are involved in the synthesis of ceramide. Overproduction of LASS3 appears to results in increases in several ceramide species, with some preference toward those having middle to long chain fatty acid acyl-CoAs. Also appears to have (dihydro)ceramide synthesis activity. LASS3 expression appears to be limited almost solely to the testis.

Synonyms: LAG1 longevity assurance homolog 3

Source

Immunogen: Synthetic peptide derived from human LASS3 protein

Product

Product Form: Affinity Purified

Formulation: Provided as a solution in Tris buffered saline with 0.08% sodium azide, 10mM MgCl2, 10mM ammonium sulfate, and 0.2% lactose.

Purification Method: Antigen Immunoaffiinity Purification

Concentration: Lot specific, see vial

Applications
Antibody can be used for Western blotting (1:400 starting dilution). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Expression appears to be solely limited to the testis.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
Mizutani, Y., et al. 'LASS3 (longevity assurance homologue 3) is a mainly testis-specific (dihydro)ceramide synthase with relatively broad substrate specificity.' Biochem. J., 2006, 398, 531-538.

Protein Reference(s)

Database Name: UniProt

Accession Number: Q8IU89

Species Accession: Human

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: X2304P Categories : Primary antibodies, Primary antibodies, Antigen Immunoaffinity Purified Polyclonal
" Western blot analysis using LAG1 longevity assurance homolog 3(Cat. X2304P) at 2.5 µg/ml on human testis lysate 14 µg/lane. Lane A] antibody alone, Lane B] antibody blocked with 40 µg blocking peptide. Lane C] conjugate alone. Visualized using Pierce West Femto substrate system. Anti Rabbit secondary used at dilution used 1:3.5K (Cat. # X1207M). Exposure for 3 minutes" " Western blot analysis using LAG1 longevity assurance homolog 3(Cat. X2304P) at 2.5 µg/ml on human testis lysate 14 µg/lane. Lane A] antibody alone, Lane B] antibody blocked with 40 µg blocking peptide. Lane C] conjugate alone. Visualized using Pierce West Femto substrate system. Anti Rabbit secondary used at dilution used 1:3.5K (Cat. # X1207M). Exposure for 3 minutes"
" Western blot analysis using LAG1 longevity assurance homolog 3(Cat. X2304P) at 2.5 µg/ml on human testis lysate 14 µg/lane. Lane A] antibody alone, Lane B] antibody blocked with 40 µg blocking peptide. Lane C] conjugate alone. Visualized using Pierce West Femto substrate system. Anti Rabbit secondary used at dilution used 1:3.5K (Cat. # X1207M). Exposure for 3 minutes"

" Western blot analysis using LAG1 longevity assurance homolog 3(Cat. X2304P) at 2.5 µg/ml on human testis lysate 14 µg/lane. Lane A] antibody alone, Lane B] antibody blocked with 40 µg blocking peptide. Lane C] conjugate alone. Visualized using Pierce West Femto substrate system. Anti Rabbit secondary used at dilution used 1:3.5K (Cat. # X1207M). Exposure for 3 minutes"