Catalogue

Background
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Synonyms: Heat shock factor protein 1

Source

Immunogen: Synthetic peptide derived from human HSF1 protein.

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Antibody can be used for immunohistochemistry (2 µg/ml) and Western blotting (1-2 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human Hepatocellular Carcinoma

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Jin, X., et al. ‘Targeted deletion of Hsf1, 2, and 4 genes in mice.’ Methods Mol. Biol., 787, 1-20 (2011) 2. Kaitsuka, T., et al. ‘Transformation of eEF1Bdelta into heat-shock response transcription factor by alternative splicing.’ EMBO Rep., 12, 673-681 (2011)

Protein Reference(s)

Database Name: SwissProt

Accession Number: Q00613

Species Accession: Human

Safety Datasheet(s) for this product: