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Rabbit anti Human FBXW7

Catalog number: X2314P
Product Type Antigen Immunoaffinity Purified Polyclonal
Units 100 µg
Host Rabbit
Species Reactivity Human
Application Western Blotting

Background
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.

Synonyms: F-box and WD-40 domain protein 7, FBXW7, CDC4

Source

Immunogen: Synthetic peptide dervied from the human FBXW7 protein

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: Lot specific, see vial

Applications
Antibody can be used for Western blotting (1:400 starting dilution). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Widely expressed in most human tissues.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Strohmaier, H., et al. 'Human F-box protein hCdc4 targets cyclin E for proteolysis and is mutated in a breast cancer cell line.; Nature 413:316-322(2001). 2. Moberg, K.H., et al. 'Archipelago regulates cyclin E levels in Drosophila and is mutated in human cancer cell lines.; Nature 413:311-316(2001).

Protein Reference(s)

Database Name: UniProt

Accession Number: Q969H0

Species Accession: Human

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: X2314P Categories : Primary antibodies, Primary antibodies, Antigen Immunoaffinity Purified Polyclonal
" Western blot analysis using FBXW7 antibody (cat. no. X2314P) on human brain lysate (cat. no. X1633C). Antibody used at 1 µg/ml dilution and is used alone (1) or incubated with blocking peptide (cat. no. X2337B) (2). Visualized using mouse anti-rabbit HRP (cat. no. X1209M) at 1:200K dilution." " Western blot analysis using FBXW7 antibody (cat. no. X2314P) on human brain lysate (cat. no. X1633C). Antibody used at 1 µg/ml dilution and is used alone (1) or incubated with blocking peptide (cat. no. X2337B) (2). Visualized using mouse anti-rabbit HRP (cat. no. X1209M) at 1:200K dilution."
" Western blot analysis using FBXW7 antibody (cat. no. X2314P) on human brain lysate (cat. no. X1633C). Antibody used at 1 µg/ml dilution and is used alone (1) or incubated with blocking peptide (cat. no. X2337B) (2). Visualized using mouse anti-rabbit HRP (cat. no. X1209M) at 1:200K dilution."

" Western blot analysis using FBXW7 antibody (cat. no. X2314P) on human brain lysate (cat. no. X1633C). Antibody used at 1 µg/ml dilution and is used alone (1) or incubated with blocking peptide (cat. no. X2337B) (2). Visualized using mouse anti-rabbit HRP (cat. no. X1209M) at 1:200K dilution."