Rabbit anti Human FAN, N-SMase Activation Associated Factor
Catalog number: X2355P| Product Type |
Polyclonal Antibody |
| Units | 100 µg |
| Host | Rabbit |
| Species Reactivity |
Human |
| Application |
Western Blotting |
Background
Novel adaptor protein which mediates the activation of sphingomyelinase by receptors such as tumor necrosis factor receptor type 1 and CD40 and subsequent apoptosis in cardiomyocytes. Inhibition of this factor may provide a novel intervention point for reducing ischemia/reperfusion injury.
Synonyms: FAN (Factor associated with neutral sphingomyelinase activation) SMAF; Neutral sphingomyelinase (N-SMase) activation associated factor
Source
Immunogen: Synthetic peptide derived from human NSMAF protein.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Antibody can be used for Western blotting (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Human testis, brain
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. O’Brien, N.W., et al. 'Factor associated with neutral sphingomyelinase activation and its role in cardiac cell death.' Circ Res. 2003 Apr 4;92(6):589-91.
2. Segui, B., et al. 'CD40 Signals apoptosis through FAN-regulated activation of the sphinogmyelin-ceramide pathway.' J Biol Chem. 1999; 274: 37251–37258
3. Adam-Klages, S., et al. 'FAN, a novel WD-repeat protein, couples the p55 TNF-receptor to neutral sphingomyelinase.' Cell. 1996; 86: 937–947.
4. Segui, B., et al. 'Involvement of FAN in TNF-induced apoptosis.' J Clin Invest. 2001; 108: 143–151.
Protein Reference(s)
Database Name: UniProt
Accession Number: Q8IW26 (Human)
Species Accession: Human
Safety Datasheet(s) for this product:
| NM_Sodium Azide |
|
" Western blot using anti nSMAF antibody (Cat. No. X2355P) on human brain lysate (A) and RMS-13 rhabdosarcoma cell lysate (B). Lysate used at 15 µg/lane. Antibody used at 1:400 dilution. Secondary antibody, mouse anti-rabbit HRP (Cat. No. X1207M), used at 1:50k dilution. Visualized using Pierce West Femto substrate system. Exposure for 5 minutes " |
" Western blot using anti nSMAF antibody (Cat. No. X2355P) on human brain lysate (A) and RMS-13 rhabdosarcoma cell lysate (B). Lysate used at 15 µg/lane. Antibody used at 1:400 dilution. Secondary antibody, mouse anti-rabbit HRP (Cat. No. X1207M), used at 1:50k dilution. Visualized using Pierce West Femto substrate system. Exposure for 5 minutes "