Catalogue
Rabbit anti Human Caspase-7
Catalog number: A135P| Isotype | IgG |
| Product Type |
Polyclonal Antibody |
| Units | 100 µg |
| Host | Rabbit |
| Species Reactivity |
Human |
| Application |
Western Blotting |
Background
Caspase-7 along with caspase 3 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 occurs in three varient forms.Exalphas rabbit anti-human caspase-7 antibody reacts with the zymogen and with the 20-23 kDa subunit of caspase-7. Cells should be pretreated with 200 ng/ml of deoxyrubicin for one half hour. Exalphas caspase-7 antibody was generated by immunizing rabbits with the aa 186-198 of human caspase-7.
Synonyms: Cysteine-requiring Aspartate Protease-7; Apoptotic protease Mch-3; ICE-like apoptotic protease 3
Source
Immunogen: Rabbits with the aa 186-198 of human caspase-7
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Applications: western blot, use at 2-10 µg/ml
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Room Temperature
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16
2. Chandler, J.M., et al. Different subcellular distribution of Caspase-3 and Caspase-7 following Fas-induced apoptosis in mouse liver. J. Bio. Chem. 1998, 273, 10815-10818
3. Behrensdorf, H.A., et al. The endothelial monocyte-activating polypeptide II (EMAP II) is a substrate for caspase-7. FEBS Lett. 2000, 466, 143-147
Protein Reference(s)
Database Name: UniProt
Accession Number: P55210
Safety Datasheet(s) for this product:
| NM_Sodium Azide |