Catalogue

Background
Acid ceramidase is a lipid hydrolyase responsible for the degradation of ceramide into sphingosine and free fatty acids within lysosomes. It can also synthesize ceramide from sphingosine and free fatty acids as well. The reverse activity is pH dependent (6.0 vs 4.5, respectively). This suggests that the enzyme may have diverse functions within cells dependent on its subcellular location and the local pH. Recent studies have shown that acid ceramidase activity is aberrantly expressed in several human cancers and that it might be a useful drug target. As an example, inhibitors of enzyme activity have been shown to slow the growth of cancer cells alone or in combination with other established, anti-oncogenic treatments. Aberrant activity has also been shown in AlzheimerÕs disease and overexpression may prevent insulin resistant (Type II) diabetes by free fatty acids.

Synonyms: N-acylsphingosine amidohydrolase (Acid ceramidase) 1; ASAH1

Source

Immunogen: Synthetic peptide derived from the C terminal region of the ASAH1 protein.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1-5 µg/ml) and Immunohistochemistry on paraffin-embedded tissues (10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human heart tissue

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Park, J.H., et al. 'Acid ceramidase and human disease.' Biochim Biophys Acta 2006; 1758:2133-2138. 2. Zeidan, Y.H., et al. 'Acid ceramidase but not acid sphingomyelinase is required for tumor necrosis factor-{alpha}-induced PGE2 production.' J. Biol. Chem. 2006; 281:24695-24703. 3. Shtraizent, N., et al. 'Autoproteolytic cleavage and activation of human acid ceramidase.' J. Biol. Chem. 2008; 283:11253-11259.

Protein Reference(s)

Database Name: UniProt

Accession Number: Q53H01-HumanQ9EQJ6-RatQ9WV54-Mouse

Species Accession: Human, Rat, Mouse

Safety Datasheet(s) for this product: