Catalogue
Rabbit anti Chlorpyrifos
Catalog number: H35sClone | Polyclonal |
Isotype | Ig |
Product Type |
Serums |
Units | 0.1 ml |
Host | Rabbit |
Application |
ELISA Immuno-affinity-chromatography |
Background
Chlorpyrifos is an insecticide belonging to the group of organophosphothioates. It is used in the culture of vegetables and in citrus orchards. Chlorpyrifos is an acetylcholinesterase inhibitor and as such having a highly toxic effect on the nervous system. There is evidence that chlorpyrifos has an effect on the foetus, which may lead to behavioural disorders.
Source
Rabbit immune serum
Immunogen: BSA-Chlorpyrifos Conjugate
Product
Each vial contains 0.1 ml rabbit polyclonal antiserum containing 0.02% sodium azide.
Specificity
Target: Chlorpyrifos, CAS no.: 2921-88-2, Solubility: Methanol, Iso-octanol, Benzene.
Species Reactivity: The specificity of the antibody was determined by measuring the cross-reactivity in ELISA for a range of compounds. Cross Reactivity Pesticides % Cross Reactivity Chlorpyrifos 100% (2,4-dichlorophenoxy)acetic acid <0.1% Pirimiphos <0.1% Deltametrin <0.1% Fenitrothion <0.1% Permethrin <0.1% Etrimphos <0.1% Atrazine <0.1% Methacrimphos <0.1% Aldicarb <0.1% Malathion <0.1% Tebuconazole <0.1%
Applications
The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC).
Suggested dilution 1:8000 in ELISA (this titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). ). Plates are coated with 400 ng/ml ovalbumin conjugated-chlorpyrifos. HRP-conjugated anti-rabbit IgG should be used as a tracer in a 1:8,000 dilution.
Storage
Store at 4 °C or -20 °C. The working antibody solution is stable for at least 7 days at 4 °C. Precautions should be taken for storage for longer periods. Problems of long term stability may occur with highly diluted solutions.
For long storage purposes in solution the addition of sodium azide to 0.02 % is advised with the appropriate precautions of use.
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.