Catalogue

Background
Bisphenol A (BPA) is predominantly used in the production of polycarbonate plastics and epoxy resins that are used in many products. It is released into the environment and food. BPA is an endocrine disruptor with estrogenic and obesogenic properties. It influences reproduction and has an epigenetic effect already in the foetus.

Source
Rabbit immune serum

Immunogen: BSA-Bisphenol Valeric Acid (BVA)

Product
Each vial contains 0.1 ml of serum containing 0.02% sodium azide.

Specificity
Immunogen: BSA-Bisphenol Valeric Acid (BVA), Target: Bisphenol A, CAS no.: 80-05-7, Solubility: Acetone, Ethanol, Ether, Benzene.

Species Reactivity: Cross Reactivity in a direct assay Molecules containing a phenolic group % Cross Reactivity Bisphenol A 100% 4,4'-(ethylidene) bisphenol 10% Bis-(4-hydroxy phenyl)-methane 1% Nonylphenol 0.1% 4-cumylphenol 1% Molecules lacking a phenolic group % Cross Reactivity Vinclozolin Pirimifos-ethyl < 0.1% 17ß-Estradiol < 0.1% Sulfadimidine < 0.1% Cross Reactivity in an indirect assay Molecules containing a phenolic group % Cross Reactivity Bisphenol A 100% 4,4'-(ethylidene) bisphenol 10% 4-cumylphenol 10% Molecules lacking a phenolic group % Cross Reactivity Vinclozolin <0.1% Pirimifos-ethyl < 0.1% 2,4 D < 0.1% Fenitrothion < 0.1% Chlorpyrifos-methyl < 0.1% Erythromycine < 0.1%

Applications
Dilution of 1/50,000 from the delivered solution. (The titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Suggested concentration to use in ELISA: 1/50,000 from the delivered solution. Plates are coated with 400 ng/ml OVA-Bisphenol Valeric Acid (BVA). HRP-conjugated anti-rabbit IgG as a tracer 1/8,000

Storage
Store at 4 °C or aliquot and store at -20 °C for longer periods. Precautions should be taken upon storage for longer periods. Problems of long term stability may occur with highly diluted solutions. For long storage purposes in solution the addition of sodium azide at 0.02 % is advised with the appropriate precautions of use.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Eline P. Meulenberg, Kees Koopal and Ria Rhemrev - Immunoassays for alkylphenolic pollutants with endocrine disrupting activity. Intern. J. Environ. Anal. Chem. Vol 85, no 12-13, 15 October-15 November 2005, 871-883