Catalogue

Background
Mucin 1, cell surface associated (MUC1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in Humans. MUC1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs.The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity.Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with colon, breast, ovarian, lung and pancreatic cancers.

Source
The 139H2 monoclonal antibody is derived by immunisation of Balb/c mice with Human milk-fat globule membranes (HMFGM).

Product
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity
Mouse monoclonal antibody has been raised anti human milk-fat globule membranes (HMFGM) to obtain reagents for mammary tumor diagnosis. This monoclonal reacts towards an peptide epitope in the repeat region of MUC1.

Applications
Antibody 139H2 is useful for ELISA, immunofluorescence, flow cytometry, immunoprecipitation, western blot, electron microscopy and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Hilkens J, Buijs F, Hilgers J, Hageman P, Calafat J, Sonnenberg A, van der Valk M. Monoclonal antibodies anti Human milk-fat globule membranes detecting differentiation antigens of the mammary gland and its tumors.

Safety Datasheet(s) for this product: