Catalogue

Background
The mammalian members of the MAGE (melanoma-associated antigen) gene family were originally described as completely silent in normal adult tissues, with the exception of male germ cells and, for some of them, placenta. By contrast, these genes were expressed in various kinds of tumors. MAGE-like genes have also been identified in non-mammalian species, like the zebrafish or Drosophila melanogaster. Although no MAGE homologous sequences have been identified in Caenorhabditis elegans, Saccharomyces cerevisiae or Schizosaccharomyces pombe, MAGE sequences have been found in several vegetal species, including Arabidopsis thaliana. The only region of homology shared by all of the members of the family is a stretch of about 200 amino acids which has been named the MAGE conserved domain. The MAGE conserved domain is usually loCated close to the C-terminal, although it can also be found in a more central position in some proteins. The MAGE conserved domain is generally present as a single copy but it is dupliCated in some proteins. It has been proposed that the MAGE conserved domain of MAGE-D proteins might interact with p75 neurotrophin or related receptors. The function of this protein is not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. The Melanoma-associated antigen is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, leukemias and lymphomas.

Source
NKI-Beteb is a Mouse monoclonal IgG2b antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Balb/c mice immunized with a smooth membrane fraction of a lymph node metastasis of melanoma.

Product
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity
NKI-Beteb reacts with Melanoma Associated Antigen, a glycoprotein of 100 + 7 kDa. Melanoma associated antigen (MAA) is dispersed in the cytoplasma of melanoma cells, and is more concentRated inside vacuoles and sometimes on the melanosomes. Occasionally the antigen is seen on the cell surface. The antigen is actively shed from living cells. Melanoma Associated Antigen 100 + 7 kDa is present in melanomas, clear cells sarcomas (melanoma of soft tissue), nevocellular nevi, and normal melanocytes. Except for one case of non Hodgkin's lymphoma in which macrophages were positive, the antigen has not been detected with other tumors or tissues observed. The antibody reacts with melanomas, clear cell sarcomas (melanoma of soft tissue), nevocellular nevi, and normal melanocytes. The antibody reacts with melanomas, clear cell sarcomas (melanoma of soft tissue), nevocellular nevi, and normal melanocytes.

Applications
NKI-Beteb is useful for immuohistochemistry on frozen and paraffin embedded sections. Optimal antibody dilution should be determined by titration.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Vennegoor C. et al.(1988), Am.J. Pathol. 130: 179-192.

Safety Datasheet(s) for this product: