Catalogue
Mouse anti Human Rb (Retinoblastoma)
Catalog number: N135M| Clone | XZ91 |
| Isotype | IgG1 |
| Product Type |
Monoclonal Antibody |
| Units | 100 µg |
| Host | Mouse |
| Species Reactivity |
Chicken Human Mink Mouse Rat |
| Application |
Immunoprecipitation Western Blotting |
Background
The immunogen for the anti-human retinoblastoma (Rb) was a recombinant protein corresponding to amino acids 393 to 572 of the human retinoblastoma sequence. Retinoblastoma encodes a phosphorylated protein p110 and binds and regulates E2F transcription factors. Rb functions as a major cell cycle regulator by its ability to repress gene transcription by all three RNA polymerases. In its hypophosphorylated form, Rb binds to a subset of E2F containing complexes converting them to repressors preventing expression of E2F target genes. These complexes repress transcription via E2F binding elements in the promotersof cell cycle control genes, such as E2F-1, c-myc, and cdc2 genes.
Synonyms: Retinoblastoma-associated protein; pRb; pp110; p105-Rb
Source
Immunogen: A recombinant protein corresponding to amino acids 387 to 928 of the human retinoblastoma sequence
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
Applications: Western Blot, Immunofluorescence
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Room Temperature
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1) E.Harlow et. al. (1991) Mol. Cell Biol., 11: 5792-5799
Protein Reference(s)
Database Name: UniProt
Accession Number: P06400 (Human)
Species Accession: Human
Safety Datasheet(s) for this product:
| NM_Sodium Azide |