Catalogue

Background
The immunogen for the anti-human retinoblastoma (Rb) was a recombinant protein corresponding to amino acids 393 to 572 of the human retinoblastoma sequence. Retinoblastoma encodes a phosphorylated protein p110 and binds and regulates E2F transcription factors. Rb functions as a major cell cycle regulator by its ability to repress gene transcription by all three RNA polymerases. In its hypophosphorylated form, Rb binds to a subset of E2F containing complexes converting them to repressors preventing expression of E2F target genes. These complexes repress transcription via E2F binding elements in the promotersof cell cycle control genes, such as E2F-1, c-myc, and cdc2 genes.

Synonyms: Retinoblastoma-associated protein; pRb; pp110; p105-Rb

Source

Immunogen: A recombinant protein corresponding to amino acids 393 to 572 of the human retinoblastoma sequence

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Applications: Immunoprecipitation (not recommended for Western blotting or immunohistochemistry).

Functional Analysis: Immunoprecipitation

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1) Hu, Q.J., et. al. 'Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.' Mol. Cell Biol. 1991, 11: 5792-5799 2) Harlow, E., 'Retinoblastoma. For our eyes only.' Nature 1992, 359, 270-271 3) Dyson, N., et al. 'Adenovirus E1A makes two distinct contacts with the retinoblastoma protein.' J. Virol. 1992, 4606-4611 4) Zarkowska, T., et al., 'Monoclonal antibodies specific for underphosphorylated retinoblastoma protein identify a cell cycle regulated phosphorylation site targeted by CDKs.' Oncogene 1997, 14, 249-254 5) Lai, A., et al. 'RBP1 Recruits the mSIN3-Histone Deacetylase Complex to the Pocket of Retinoblastoma Tumor Suppressor Family Proteins Found in Limited Discrete Regions of the Nucleus at Growth Arrest.

Protein Reference(s)

Database Name: UniProt

Accession Number: P06400 (Human)

Species Accession: Human

Safety Datasheet(s) for this product: