Mouse anti Human Prostaglandin-E2 receptor EP2Catalog number: X1491M
Prostaglandins (PG's) are produced by the metabolism of arachidonic acid. PGE-2 is one of the five physiologically significant prostanoids known. ItÕs wide spectrum of physiologic and pharmacologic effects in various tissues are mediated through binding to the PGE-2 receptors (EP1, EP2, EP3 & EP4). These include effects on the immune, endocrine, cardiovascular, renal and reproductive systems as well as smooth muscle. It is also one of the most abundant of the prostanoid family in the brain where it plays an important role in many neural functions, particularly in newborn babies, and as a mediator of inflammation. PGE-2 signals through a family of G-protein coupled receptors known as EP receptors. There are 4 subtypes of EP receptors, known as EP1, EP2, EP3 and EP4. EP2 receptors are 358 amino acid proteins with a short third intracellular loop. EP2 receptors stimulate adenylyl cyclase by their coupling to Gs and do not undergo PGE-2-induced internalization. The EP2 receptors is involved with the contration and relaxation of smooth muscle tissue. These receptors are mainly localized in lung and placental tissues and in smooth muscle.
Synonyms: Prostaglandin E2 Receptor Subtype EP2; Provided under license from Allergan, Inc. United States Patent No. 5,716,835
Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human EP2 receptor protein and mouse myeloma cells.
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form: Unconjugated
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
This antibody can be used for Western blot analysis (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Porcine brain lysate
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Bhattacharya, M., et al. Nuclear prostaglandin receptors. Gene Ther. Mol. Biol. 1999, 4, 323-338 2. Desai, S., et al. Comparison of agonist-induced internalization of the human EP2 and EP4 prostaglandin receptors: role of the carboxyl terminus in EP4 receptor sequestration. Mol. Pharmacol. 2000, 58, 1279-1286 3. Morath, R., et al. Immunolocalization of the four prostaglandin E2 receptor proteins EP1, EP2, EP3 and EP4 in human kidney. J. Am. Soc. Nephrol. 1999, 10, 1851-1860 4. Fedyk, E.R., et al. Prostaglandin E2 receptors of the EP2 and EP4 subtypes regulate activation and differentiation of mouse B lymphocytes to IgE-secreting cells. Proc. Natl. Acad. Sci. USA 1996, 93, 10978-10983 5. Najarian, T., et al. Preservation of neural function in the perinate by high PGE(2) levels acting via EP(2) receptors. J. Appl. Physiol. 2000, 89, 777-784.
Database Name: SwissProt
Accession Number: Q62928
Species Accession: Rat
Safety Datasheet(s) for this product:
" Western blot analysis using EP2 antibody on bovine brain lysate at 1 µg/ml."