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Mouse anti Human p16 - ink4a

Catalog number: C170M
Clone DCS50.1
Isotype IgG1
Product Type Monoclonal Antibody
Units 100 µg
Host Mouse
Species Reactivity Human
Application Immunohistochemistry
Immunoprecipitation
Western Blotting

Background
The immunogen for the anti-human p16 was a recombinant full length p16 protein recognizing a 16kDa protein. The p16ink4a tumor suppressor protein shows no cross reactivity with the closely related inhibitors such as p15ink4b and p18ink4c. p16ink4a is a specific inhibitor of cdk4/cdk6, and is a tumor suppressor involved in the pathogenesis of a number of malignancies including melanoma and lung carcinoma. It can be used in immunohistochemistry as an indicator of HPV infection in cervical and head and neck (pre)malignancies.

Synonyms: hp16; p16 ink4a; Cyclin dependent kinase inhibitor 2A, isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; CDK4I

Source

Immunogen: A recombinant full length p16 protein recognizing a 16kDa protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Applications: Western Blot, Immunoflurescence, Immunoprecipitation (co-precipitates cdk4/cdk6)

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1) J. Lukas et.al (1995) Nature., 375,503-506 2) L. Aagaard et.al. (1995), Intl. J. of Cancer 61(1) 115-120

Protein Reference(s)

Database Name: UniProt

Accession Number: P42771 (Human)

Species Accession: Human

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: C170M Categories : Primary antibodies, Primary antibodies, Monoclonal Antibody
Immunohistochemical staining of PPFE bladder tumor tissue using p16-INK4a antibody (Cat. No. C170M). Immunohistochemical staining of PPFE bladder tumor tissue using p16-INK4a antibody (Cat. No. C170M).
Figure 2. Immunohistochemical staining of formalin fixed, paraffin embedded tissue from an HPV infected preneoplastic lesion in the uterine cervix using p16-INK4a antibody (Cat. No. C170M). Antigen retreival was performed with Tris/EDTA buffer pH 9. Figure 2. Immunohistochemical staining of formalin fixed, paraffin embedded tissue from an HPV infected preneoplastic lesion in the uterine cervix using p16-INK4a antibody (Cat. No. C170M). Antigen retreival was performed with Tris/EDTA buffer pH 9.
Immunohistochemical staining of PPFE bladder tumor tissue using p16-INK4a antibody (Cat. No. C170M).

Immunohistochemical staining of PPFE bladder tumor tissue using p16-INK4a antibody (Cat. No. C170M).

Figure 2. Immunohistochemical staining of formalin fixed, paraffin embedded tissue from an HPV infected preneoplastic lesion in the uterine cervix using p16-INK4a antibody (Cat. No. C170M). Antigen retreival was performed with Tris/EDTA buffer pH 9.

Figure 2. Immunohistochemical staining of formalin fixed, paraffin embedded tissue from an HPV infected preneoplastic lesion in the uterine cervix using p16-INK4a antibody (Cat. No. C170M). Antigen retreival was performed with Tris/EDTA buffer pH 9.