Catalogue
Mouse anti Human p13
Catalog number: X1235M| Clone | BM-3 |
| Isotype | IgG1 |
| Product Type |
Monoclonal Antibody |
| Units | 100 µg |
| Host | Mouse |
| Species Reactivity |
Human |
| Application |
Flow Cytometry Immunohistochemistry (frozen) |
Background
Used as a marker for human myeloid cells. p13 protein is a nuclear antigen expressed in human granulocytes and monocytes in lymphoid and non-lymphoid tissues and is expressed during the early phases of myeloid differentiation.
Source
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with nuclei from Pokeweed mitogen stimulated with peripheral blood lymphocytes and mouse myeloma cells.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Antibody can be used for immunohistochemistry on frozen tissues sections and flow cytometry. Not for use on paraffin embedded tissue sections. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: HL-60 cells
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. Epstein, A.L. and Clevenger, C.V. 'Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immunobiochemical methods using monoclonal antibodies.' Progress in Nonhistone Protein Research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp. 117-137
2. Murao, S., et al. 'Expression of maturation-specific nuclear antigens in differentiating human myeloid leukemia cells.' Cancer Research 1985: 45:791-795
Protein Reference(s)
Database Name: SwissProt
Accession Number: P56278
Species Accession: Human
Safety Datasheet(s) for this product:
| NM_Sodium Azide |