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Mouse anti Human Myogenin

Catalog number: X1415M
Clone F12B
Isotype IgG1
Product Type Monoclonal Antibody
Units 100 µg
Host Mouse
Species Reactivity Human
Mouse
Rat
Application ELISA
Gel Supershift
Immunofluorescence
Immunohistochemistry (paraffin)
Immunoprecipitation
Western Blotting

Background
Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.

Synonyms: Myogenin, Myogenic factor 4, Myf-4

Source
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.

Product
X1415M, clone F12B recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation of protein-DNA complexes (2 µg/mg), Western blotting (1 µg/ml) and is the antibody of choise for immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Rhabdomyosarcoma and Wlim's tumors

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Wright WE, et al. Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interaction. Dev Genet 19: 131-138 (1996). 2. Wright WE, et al. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell 1989; 56:607. 3. Aurade F, et al. Myf5, MyoD, myogenin and MRF4 myogenic derivatives of the embryonic mesenchymal cell line C3H10T1/2 exhibit the same adult muscle phenotype. Differentiation 1994; 55:185.

Protein Reference(s)

Database Name: UniProt

Accession Number: P15173 (Human) P12979 (Mouse) P20428 (Rat)

Species Accession: Human, Rat, Mouse

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: X1415M Categories : Primary antibodies, Primary antibodies, Monoclonal Antibody
Figure 1. Immunohistochemical staining using myogenin antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma. Figure 1. Immunohistochemical staining using myogenin antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma.
Figure 2: Immunoreactivity of X1415M, the mouse anti human myogenin in nuclei of formaldehyde fixed C2C12 mouse muscle myoblast cells in culture (high magnification). Figure 2: Immunoreactivity of X1415M, the mouse anti human myogenin in nuclei of formaldehyde fixed C2C12 mouse muscle myoblast cells in culture (high magnification).
Figure 1. Immunohistochemical staining using myogenin antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma.

Figure 1. Immunohistochemical staining using myogenin antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma.

Figure 2: Immunoreactivity of X1415M, the mouse anti human myogenin in nuclei of formaldehyde fixed C2C12 mouse muscle myoblast cells in culture (high magnification).

Figure 2: Immunoreactivity of X1415M, the mouse anti human myogenin in nuclei of formaldehyde fixed C2C12 mouse muscle myoblast cells in culture (high magnification).