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Mouse anti Human LEF-1 beta catenin

Catalog number: T100MS
Clone REMB1
Isotype IgG1
Product Type Monoclonal Antibody
Units 100 µg
Host Mouse
Species Reactivity Human
Mouse
Application Immunofluorescense
Immunohistochemistry (paraffin)
Western Blotting

Background
Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on Western blots as smaller immunoreactive forms.

Synonyms: lef-1, mouse anti human lymphoid enhancer factor-1

Source
The immunogen used for production of the mouse monoclonal anti-LEF-1 antibody was a full length protein.

Product
Clone REMB1 recognizes the beta-catenin binding domain of LEF-1.

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1-5 µg/ml), immunofluorescence and in immunohistochemistry on paraformaldehyde-fixed cells and paraffin embedded tissues (1:1000 or 1 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Jurkat cell lysate

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
Hsu SC; Galceran J; Grosschedl R. 'Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin.' Mol Cell Biol, 18(8):4807-18 1998 IHC Referene: Dorfman, David M. et al. , Loss of Expression of the WNT/Beta Catenin Signaling Pathway Transcription Factors Lymphoid Enhancer Factor-1 (LEF-1) and T Cell factor-1 (TCF-10 in a Subset of Peripheral T Cel Lymphomas, The American Journal of Pathology, 2003, pp 1538-1544.

Protein Reference(s)

Database Name: UniProt

Accession Number: Q9UJU2

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: T100MS Categories : Primary antibodies, Primary antibodies, Monoclonal Antibody
Figure 1. Immunofluorescence staining of Cos -1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF -1. Cells were stained with Exalpha’s LEF-1 antibody, clone REMB1 cat. # T100M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1 Figure 1. Immunofluorescence staining of Cos -1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF -1. Cells were stained with Exalpha’s LEF-1 antibody, clone REMB1 cat. # T100M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1
Figure 1. Immunofluorescence staining of Cos -1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF -1. Cells were stained with Exalpha’s LEF-1 antibody, clone REMB1 cat. # T100M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1

Figure 1. Immunofluorescence staining of Cos -1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF -1. Cells were stained with Exalpha’s LEF-1 antibody, clone REMB1 cat. # T100M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1