Catalogue

Background
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.This antibody reacts specifically with human keratin 14 by immunoblotting. In tissue sections, it provides a positive reaction on basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas.

Source

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide conjugated to KLH corresponding to the carboxy terminal sequence of human cytokeratin 14 (KVVSTHEQVLRTKN) and mouse myeloma cells.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Detects human cytokeratin 14 by Western blot at 50 kDa. Does not react with other cytokeratins or other proteins. Also works for immunohistochemistry using frozen tissues. Does not work with paraffin embedded tissues. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Moll, R., et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982, 31, 11-24 2. Ivanyi, D., et al. Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. American Journal of Veterinary Research, 1992, 53, 304-314.

Protein Reference(s)

Database Name: SwissProt

Accession Number: P02533

Species Accession: Human

Safety Datasheet(s) for this product: