Catalogue
Mouse anti Human Heat Shock Protein 27
Catalog number: X1264M| Clone | G3.1 |
| Isotype | IgG1 |
| Product Type |
Monoclonal Antibody |
| Units | 100 µg |
| Host | Mouse |
| Species Reactivity |
Human |
| Application |
ELISA Enzyme Immunoassay Immunocytochemistry Immunohistochemistry (frozen & paraffin) Immunoprecipitation Western Blotting |
Background
HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. HSP27 synthesis is induced by elevated temperature as well as estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosphilia HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
Source
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with partially purified HSP27 derived from MCF-7 cytosol and mouse myeloma cells.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
This antibody can be used for immunohistochemistry and immunocytochemistry on frozen and paraffin-embedded tissue sections as well as for ELISA, immunoprecipitation and Western blot. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. Adams, D.J., et al. 'Detection of a Mr 24,000 estrogen-regulated protein in human breast cancer by monoclonal antibodies.' Cancer Res. 1983, 43, 4297-4301.
2. Moretti-Rojas, I., et al., 'A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells.' Breast Cancer Res. Treat. 1988, 11, 155-163.
3. Hino, M., et al. 'Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.' Biochem. Biophys. Res. Commun. 2000, 271, 164-169.
4. Ciocca, D.R. and Luque, E.H. 'Immunological evidence for the identity between the hsp27 estrogen-regulated heat shock protein and the p29 estrogen receptor-associated protein in breast and endometrial cancer.' Breast Cancer Res. Treat. 1991, 20, 33-42.
Protein Reference(s)
Database Name: SwissProt
Accession Number: P04792
Species Accession: Human
Safety Datasheet(s) for this product:
| NM_Sodium Azide |
|
Figure 1. Formalin-fixed, paraffin-embedded human breast carcinoma stained with Heat Shock Protein 27 (Cat. #X1264M) using peroxidaseconjugate and DAB chromogen. Note intense cytoplasmic staining of tumor cells. |
Figure 1. Formalin-fixed, paraffin-embedded human breast carcinoma stained with Heat Shock Protein 27 (Cat. #X1264M) using peroxidaseconjugate and DAB chromogen. Note intense cytoplasmic staining of tumor cells.