Mouse anti Human FetoproteinCatalog number: X1841M
Alpha-Feto Protein (AFP) is a protein produced by the fetus, which is excreted into the amniotic fluid and into the mother's bloodstream through the placenta. The amount of AFP, both in the maternalÕs blood and in the amniotic fluid, at particular periods during the pregnancy, may be associated with the presence of neural tube defects or chromosomal problems in the baby. AFP synthesis occurs in tissues including the gut, liver and fetal yolk sac. AFP expression may be seen in germ cell neoplasma and in liver carcinoma.
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with purified human alpha-fetoprotein and mouse myeloma cells.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Antibody can be used for immunohistochemistry on formalin-fixed, paraffin embedded tissues (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Immunohistochemistry
Positive Control: HepG2 cells
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Yazova AK; et al. 'Human alpha-fetoprotein epitopes as revealed by monoclonal antibodies.' Immunology Letters, 1990 Sep, 25(4):325-30. 2. Sato, Y., et al. 'Human mesenchymal stem cells xenografted directly to rat liver are differentiated into human hepatocytes without fusion.' Blood, 106, 756-763 (2005).
Safety Datasheet(s) for this product:
|Western blot using Exalpha’s X1841M anti AFp monoclonal antibody at 4 ug/ml on HepG2 cell lysate (10 ug/lane). Anti-Mouse HRp was used at 1:75k. Blots were developed using Pierce’s Super Signal West Femto. Exposure was 1 2 minutes.|
Western blot using Exalpha’s X1841M anti AFp monoclonal antibody at 4 ug/ml on HepG2 cell lysate (10 ug/lane). Anti-Mouse HRp was used at 1:75k. Blots were developed using Pierce’s Super Signal West Femto. Exposure was 1 2 minutes.