Catalogue
Mouse anti Human Estrogen Receptor
Catalog number: X1262M| Clone | AER314 |
| Isotype | IgG1 |
| Product Type |
Monoclonal Antibody |
| Units | 100 µg |
| Host | Mouse |
| Species Reactivity |
Human Mouse |
| Application |
ELISA Immunohistochemistry (frozen & paraffin) Immunoprecipitation Western Blotting |
Background
The human estrogen receptor has a molecular weight of 67 kDa and consists of 6 functional domains (domains A-F). Functional mapping of the estrogen receptor has determined a transcriptional promoting activity in the A/B domain. The hormone-binding domain (E domain) is located towards the carboxy terminal, whereas the DNA-binding domain (C domain) is found in the central portion of the molecule. It has been speculated that the presence in breast cancer cells of truncated forms of the estrogen receptor lacking the hormone-binding domain might promote the uncontrolled growth of the tumor.
Source
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with purified estrogen receptors from calf uterus tumor and mouse myeloma cells.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
Antibody can be used for staining of human and mouse frozen and paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. Abbondanza, E., et al. 'Characterization and epitope mapping of a new panel of monoclonal antibodies to estradiol receptor.' Steroids 1993, 58, 4-12.
Protein Reference(s)
Database Name: SwissProt
Accession Number: P03372
Species Accession: Human
Safety Datasheet(s) for this product:
| NM_Sodium Azide |