Catalogue

Background
The reactivity of this preparation of two monoclonal antibodies is restricted to a surface determinant on the kappa light chain. It is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500; in ELISA from 1:1,000 upwards; in Western blotting from 1:2.000 upwards. These data should be interpreted as general recommendations only.

Source
Highly purified Bence Jones kappa proteins isolated from pooled human urine.

Product
No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.

Product Form: Biotin

Formulation: No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Purification Method: ProteinA/G affinity purification

Specificity
Biotin-conjugated purified monoclonal Mouse antibody to Human Ig kappa chain, free and bound

Species Reactivity: The antibody does not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

Applications
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Storage
The lyophilized product may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.

Shipping Conditions: The lyophilized product is shipped at ambient temperature.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.