High Quality Antibody Reagents

Catalogue

Print Friendly and PDF

Mouse anti Human bcl-xl

Catalog number: A110M
Clone 2H12
Isotype IgG2a
Product Type Monoclonal Antibody
Units 100 µg
Host Mouse
Species Reactivity Human
Mouse
Rat
Application Western Blotting

Background
Overexpression of bcl-xl to other bcl-2 members is believed to promote cell survival. The ratio of bcl-xl to other family members is believed to modulate the apoptotic process.

Synonyms: bcl-xl, mouse anti human bcl xl, bclxl

Source
Mice were immunized with a synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence.

Immunogen: A synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence

Product
Provided as solution in phosphate buffered saline.

Product Form: Unconjugated

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Application: Western Blot at 1-5 ug/ml.

Functional Analysis: Western Blotting

Positive Control: Human, mouse and rat thymocytes

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1) L.H. Boise, et. al. (1993) Cell, 74:597-608. 2) Y.T. Hsu et. al. (1997) J. Biol. Chem., 272(21): 13829-13834 3) Y. T. Hsu et. al (1997) Proc. Natl. Acad. Sci. USA, 94: 3668-3672

Protein Reference(s)

Database Name: UniProt

Accession Number: Q07817

SKU: A110M Categories : Primary antibodies, Primary antibodies, Monoclonal Antibody
Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. Exalpha’s anti-Bcl-XL antibody was used at 1 µg/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody. Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. Exalpha’s anti-Bcl-XL antibody was used at 1 µg/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.
Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. Exalpha’s anti-Bcl-XL antibody was used at 1 µg/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.

Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. Exalpha’s anti-Bcl-XL antibody was used at 1 µg/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.