Catalogue

Background
SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Beta-SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas alpha-SNAP is expressed in all mammalian tissues, beta-SNAP is expressed only in brain.

Synonyms: alpha/beta SNAP, mouse anti alpha soluble NSF attachment protein,SNAPA

Source
Monoclonal antibody 15D4 obtained immunization of Balb/C mice with recombinant human alpha -SNAP

Immunogen: Balb/C mice were immunized with recombinant human alpha -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.

Product
100 µg monoclonal antibody in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol. No preservatives have been added.

Product Form: Unconjugated

Formulation: 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol. No preservatives have been included in the antibody solution.

Purification Method: The antibody was purified to >95% homogeneity by standard chromatographic techniques

Concentration: See vial for concentration

Specificity
Human alpha -SNAP

Applications
Optimal concentration should be evaluated by serial dilutions. Clone 4E4 anti-?SNAP specifically recognizes ?-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and does not recognize ?-SNAP Application: A dilution of 0.5-5 ug/ml is suggested for western blot and 2-10 ug/ml for immunoprecipitation.

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

Protein Reference(s)

Database Name: UniProt

Accession Number: P54920 (Human)

Species Accession: Human

Safety Datasheet(s) for this product: