Catalogue

Background
The major histocompatibility complex (MHC) is a cluster of genes that play an important role in the immune response. In humans, this complex is referred to as the Human Leucocyte Antigen (HLA) system. There are three major HLA class I genes which are HLA-A, HLA-B and HLA-C. These are highly polymorphic and thousands of alleles are known to exist. The HLA-A, B and C gene products are heterodimeric cell surface glycoproteins which all consist of a 40-45kD MHC-encoded alpha chain and a non-covalently linked non-MHC encoded light chain (b2-microglobulin) of 12kDa. These proteins are found on the surface of almost all nucleated cells and their role is to present peptides, produced by intracellular protein degradation, to cytotoxic T lymphocytes and natural killer cells.

Source
BRA-23/9 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma cells with cells from a mouse immunized with REH cells, a human pre-B cell leukaemia cell line.

Product
Each vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.

Formulation: Each vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.

Specificity
The antibody BRA-23/9 is directed against a non-polymorphic determinant of human HLA Class I. The antibody has not been tested for cross reactivity with other species.

Applications
The BRA-23/9 antibody is suitable for the detection of HLA Class I antigen by flow cytometry and Immunohistochemistry on frozen tissues, for example from human tonsil, lymph nodes, as well as lymphocytes Optimal antibody dilutions for the different applications should be determined by titration.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Tanaka J, Toubai T, Tsutsumi Y, Miura Y, Kato N, Umehara S, Kahata K, Mori A, Toyoshima N, Ota S, Kobayashi T, Kobayashi M, Kasai M, Asaka M, Imamura M. (2004). Cytolytic activity and regulatory functions of inhibitory NK cell receptor-expressing T cells expanded from granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cells. Blood 104:768-74. 2. 2. Tóth J, Kubes M. (1993). Masking of HLA class I molecules expressed on K-562 target cells can restore their susceptibility to NK cell cytolysis. Immunobiology. 188:134-44.

Protein Reference(s)

Database Name: UniProt

Accession Number: UniProt: P30443 (Human)

Species Accession: Human

Safety Datasheet(s) for this product: