Catalogue
Mouse anti HA Tag
Catalog number: X1000Clone | 12CA5 |
Isotype | IgG2b |
Product Type |
Monoclonal Antibody |
Units | 100 µg |
Host | Mouse |
Application |
Enzyme Immunoassay Immunohistochemistry Immunoprecipitation Western Blotting |
Background
An HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein when doing binding studies with transfected cells.
Synonyms: Mouse Anti HA, hemaglutinin
Source
Immunogen: Balb/C mice were immunized with a 9 amino acid petide to influenza virus hemagglutinin
Product
The product contains 100 µg of purified antibody in PBS containing 0.08% sodium azide
Product Form: Unconjugated
Purification Method: Protein A/G Chromatography
Purity: >95% by SDS Page
Concentration: See vial for concentration
Applications
Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: HA taged proteins
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Protein Reference(s)
Database Name: UniProt
Accession Number: Q3TBD2
Safety Datasheet(s) for this product:
NM_Sodium Azide |
Western blot using anti-HA tag antibody (Cat. No. X1000) on HEK293 cells (1) and HEK293 cells expressing HA-tagged Rem protein at 1:1000 dilution. |
Western blot using anti-HA tag antibody (Cat. No. X1000) on HEK293 cells (1) and HEK293 cells expressing HA-tagged Rem protein at 1:1000 dilution.