Catalogue
Mouse anti FITC (Fluorescein isothiocyanate)
Catalog number: X1542M| Clone | F4/1 |
| Isotype | IgG1 |
| Product Type |
Monoclonal Antibody |
| Units | 100 µg |
| Host | Mouse |
| Application |
ELISA Flow Cytometry Western Blotting |
Background
The F4/1 antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxyfluorescein. Sensitivity of the FITC-anti- FITC system is reportedly similar to that of the biotin-avidin system. When conjugated to biotin or enzyme-labeled, this antibody is also suitable for the conversion of fluorescein-labeled detection into immunoenzymatic assays.
Source
F4/1 is a hybridoma formed by fusing SP-2/0 Agl4 cells with spleen cells of a BALB/c mouse that had been immunised with FITC-KLH.
Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form: FITC
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
This antibody can be used for Western blotting (1-2 µg/ml), immunohistochemistry (frozen and formalin/paraffin tissues, 2-4 µg/ml) and flow cytometry. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Antibodies labeled with FITC
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. Balogh P; et al. 'Hapten mediated identification of cell membrane antigens using an anti-FITC monoclonal antibody.' J. Immunol. Methods 1994, 169, 35-40.
Safety Datasheet(s) for this product:
| NM_Sodium Azide |