Catalogue

Background
Estrogen receptor (ER) is a hormone binding molecule, which belongs to the nuclear hormone family of intracellular receptors. Estrogen receptors exist as alpha and beta forms encoded by separate genes ESR1 and ESR2. ERα and ERβ share a high degree of sequence homology and are activated by the hormone 17β-estradiol and related compounds, including phytoestrogens and xenoestrogens. Both ERα and ERβ function as transcription factors which mediate many of the biological effects of estradiol at the level of gene regulation. Both receptors and are involved in the normal development and function of numerous tissues. Studies also suggest that ER’s may be involved in a range of pathological conditions including cancers, neurodegenerative diseases and cardiovascular disease.

Source
AER304 is a mouse monoclonal IgG1 antibody derived by fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from a BALB/c mouse, immunized with estrogen receptor protein purified from calf uterus.

Product
Each vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.

Formulation: Each vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.

Specificity
The antibody AER304 is directed against estrogen receptor alpha (ERα) and recognises amino acids residues 120 – 170, an epitope located in the B domain of ERα.

Applications
The AER304 antibody is suitable for the detection of estrogen receptor alpha by western blotting, immunoprecipitation and immunocytochemistry. In western blotting, the antibody recognises a doublet of approximately 67 kDa in non-reducing conditions. Optimal antibody dilutions for the different applications should be determined by titration.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Abbondanza C. et al. (1993) Characterization and epitope mapping of a new panel of monoclonal antibodies to estradiol receptor. Steroids. 58: 4-12.

Protein Reference(s)

Database Name: UniProt

Accession Number: UniProt: P03372 (Human)

Species Accession: Human

Safety Datasheet(s) for this product: