Catalogue
Human Serum albumin
Catalog number: HuAlb| Product Type |
Proteins & Peptides |
| Units | 10 mg |
| Application |
Western Blotting |
Background
Albumin is a stable small polypeptide with a strong antigenicity. It has a molecular weight of 69,000 and an isoelectric point of 5.7. Purified human albumin may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
Product
Chromatographically purified human albumin. Lyophilized, stable white powder. No preservative added. After opening the vial, the lyophilized content is reconstituted by adding 2 ml of sterile distilled water, mixed gently by inversion until complete dissolution is obtained.
Formulation: Lyophilized, stable white powder. No preservative added.
Purification Method: The protein is isolated from serum by sequential precipitation and further purified by ion exchange chromatography. The identity is confirmed and tested by immunoelectrophoresis and radial immuno¬diffusion using antisera to total serum proteins and albumin.
Purity: Not less than 98% as determined by agar-gel electrophoresis. Tested in a concentration of 86 mg/ml at the level of sensitivity of the applied immunoprecipitating techniques using antisera against total serum proteins, transferrin and C3c, this product contains a trace of transferrin
Specificity
Chromatographically purified human albumin.
Applications
Purified human albumin may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immuno¬electrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
Storage
This product is shipped lyophilized at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
Shipping Conditions: Ship at ambient temperature.
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.