Catalogue
Degenerative Spermatocyte Homolog 2
Catalog number: X2358B| Product Type |
Blocking Peptide |
| Units | 50 µg |
| Host | Rabbit |
| Species Reactivity |
Human |
| Application |
Western Blotting |
Background
Bifunctional enzyme which acts as both a sphingolipid delta(4)-desaturase and a sphingolipid C4-hydroxylase. Up-regulated during keratinocyte differentiation. Not expressed at day 0 or day 3 after differentiation, detected on day 6 and increases by day 9. Belongs to the fatty acid desaturase family. DEGS subfamily. Highly expressed in skin, intestine and kidney.
Synonyms: DEGS2; Sphingolipid delta(4)-desaturase/C4-hydroxylase DES2
Source
Immunogen: Synthetic peptide derived from human DEGS2 protein.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline
Concentration: See vial for concentration
Applications
For use with DEGS2 polyclonal antibodies (Cat. No. X2356P & X2357P).
Functional Analysis: Western Blotting
Positive Control: Highly expressed in skin, intestine and kidney.
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. Mizutani, Y., et al. 'Identification of the human sphingolipid C4-hydroxylase, hDES2, and its up-regulation during keratinocyte differentiation.; FEBS Lett. 563:93-97(2004).
2. Omae, F., et al. 'DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestine.' Biochem J. 2004 May 1;379(Pt 3):687-95.
3. Omae, F. et al. 'Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2.' FEBS Lett. 2004 Oct 8;576(1-2):63-7.
4. Enomoto, A., et al. 'Dihydroceramide:sphinganine C-4-hydroxylation requires Des2 hydroxylase and the membrane form of cytochrome b5.' Biochem J. 2006 Jul 15;397(2):289-95.
Protein Reference(s)
Database Name: UniProt
Accession Number: Q6QHC5 (Human)
Species Accession: Human