Chicken anti Staphylococcal Protein A, conjugated with HRP
Catalog number: APA-HRP| Product Type |
Primary antibodies |
| Units | 0.5 mg |
| Host | Chicken |
| Species Reactivity |
Bacteria |
| Application |
ELISA Western Blotting |
Source
Chickens were immunized with purified recombinant protein A. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction purified. Antibodies were immunoaffinity-purified from a protein A agarose column and conjugated to horseradish peroxidase (HRP).
Product
Product Form: HRP conjugated
Formulation: Phosphate Buffered Saline‚ 50% Glycerol with 0.1% 2-Chloroacetamide
Purification Method: Egg-yolk derived IgY Affinity Purified
Concentration: See vial for concentration.
Applications
This antibody is suitable for ELISA and immuoblotting. Recommended starting dilutions for ELISA capture is 1:100 - 1:500 and for blotting, 1:5,000 - 1:20,000.
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles.
Shipping Conditions: Ship at ambient temperature
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Freiherr von Roman M1, Koller A, von Rüden D, Berensmeier S. 2014. Improved extracellular expression and purification of recombinant Staphylococcus aureus protein A. Protein Expr Purif. 93:87-92
Safety Datasheet(s) for this product:
| NM_Glycerol |
| NM_Antibodies-with 2-Chloroacetamide |