Catalogue
Chicken anti Fenitrothion
Catalog number: C3p| Clone | Polyclonal |
| Isotype | IgY |
| Product Type |
Primary Antibodies |
| Units | 1 ml |
| Host | Chicken |
| Application |
ELISA Immuno-affinity-chromatography |
Background
Fenitrothion is an insecticide belonging to the group of organophosphothioates. It is found on fruits, vegetables and in the environment. Application is also in the storage of grain products. It has adverse effects on algae and mammals due to its biological activity as acetylcholine esterase inhibitor. In water organisms it may have and endocrine (thyroidal) effect.
Source
Chicken egg yolk, purified by PEG extraction
Immunogen: BSA-Fenitrothion Conjugate
Product
Each vial contains 1 ml 5mg/ml of purified (PEG) polyclonal IgY antibody in 0.1 M PBS pH 7.2 containing 0.02% sodium azide.
Specificity
Target: Fenitrothion, CAS no.: 122-14-5, Solubility: Organic solvents
Species Reactivity: Cross Reactivity Pesticides % Cross Reactivity Fenitrothion 100% 2-4 D <0.1% Chloropiriphos <0.1% Deltametrin <0.1% Pirimiphos <0.1% Permethrin <0.1% Etrimphos <0.1% Atrazine <0.1% Methacrimphos <0.1% Aldicarb <0.1% Malathion <0.1% Tebuconazole <0.1%
Applications
Dilution of 1/10,000 from the delivered solution. (The titer is defined as the dilution that gives 50 % of the maximum absorbance when tested with ELISA). Suggested concentration to use in ELISA: 1/10,000 from the delivered solution. Plates are coated with 400 ng/ml OVA-conjugated fenitrothion. HRP-conjugated anti-chicken-IgG as a tracer 1/8,000.
Storage
Store at 4 °C or aliquot and store at -20 °C for longer periods.
Precautions should be taken upon storage for longer periods. Problems of long term stability may occur with highly diluted solutions.
For long storage purposes in solution the addition of sodium azide at 0.02 % is advised with the appropriate precautions of use.
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.